CoraLite®555-conjugated Cytokeratin 20 Monoclonal antibody

Cytokeratin 20 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4D10A4

Cat no : CL555-60183

Synonyms

CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT



Tested Applications

Positive IF detected inhuman colon tissue, HT-29 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60183 targets Cytokeratin 20 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11336
Full Name keratin 20
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC031559
Gene Symbol KRT20
Gene ID (NCBI) 54474
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Protocols

Product Specific Protocols
IF protocol for CL555 Cytokeratin 20 antibody CL555-60183Download protocol
Standard Protocols
Click here to view our Standard Protocols