Filter: AllWBIHCIF-PIF/ICC

WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 10x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 40x lens) showing negative staining.. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human urothelial carcinoma using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IHC staining of human urothelial carcinoma using 60183-1-Ig (same clone as 60183-1-PBS)
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IF Staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:400 and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Collagen Type III (N-terminal) antibody (22734-1-AP, green), CoraLite®594 CD68 antibody (CL594-25747, red). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:400 and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Collagen Type III (N-terminal) antibody (22734-1-AP, green), CoraLite®594 CD68 antibody (CL594-25747, red). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.
IF Staining of HT-29 using 60183-1-Ig (same clone as 60183-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

Cytokeratin 20 Monoclonal antibody, PBS Only

Cytokeratin 20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 60183-1-PBS

Clone No.4D10A4

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60183-1-PBS targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag11336 Product name: Recombinant human KRT20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 74-424 aa of BC031559 Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI Predict reactive species
Full Name	keratin 20
Calculated Molecular Weight	424 aa, 48 kDa
Observed Molecular Weight	46 kDa
GenBank Accession Number	BC031559
Gene Symbol	Cytokeratin 20
Gene ID (NCBI)	54474
RRID	AB_10858399
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P35900
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.