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MCL1 Antibody

MCL1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E3C2

Cat no : 66026-1-Ig

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM



Tested Applications

Positive WB detected inJurkat cells, Raji cells, HeLa cells, A431 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 66026-1-Ig immunogen is Fusion Protein MCL1 protein expressed in E. coli.

Reactivity human
Cited Species human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MCL1 fusion protein Ag10609
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Calculated molecular weight 350aa,37 kDa
Observed molecular weight 37 kDa
GenBank accession number BC107735
Gene symbol MCL1
Gene ID (NCBI) 4170
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.

Protocols

Product Specific Protocols
WB protocol for MCL1 antibody 66026-1-IgDownload protocol
IHC protocol for MCL1 antibody 66026-1-IgDownload protocol
IF protocol for MCL1 antibody 66026-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Reprod Sci

Downregulation of MicroRNA-125a in Placenta Accreta Spectrum Disorders Contributes Antiapoptosis of Implantation Site Intermediate Trophoblasts by Targeting MCL1.

Authors - Yongzhong Gu
humanWB

Oncol Lett

Reduced ubiquitin-specific protease 9X expression induced by RNA interference inhibits the bioactivity of hepatocellular carcinoma cells.

Authors - Huiwen Hu
humanWB

Mol Med Rep

Sox2 inhibits Wnt-β-catenin signaling and metastatic potency of cisplatin-resistant lung adenocarcinoma cells.

Authors - Jinxi He
humanWB

J Bioenerg Biomembr

Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer.

Authors - Yiming Sun
humanWB

Reprod Biol

Hsa-miRNA-125b may induce apoptosis of HTR8/SVneo cells by targeting MCL1.

Authors - Yongzhong Gu
humanWB

Dig Dis Sci

Reduced high-mobility group box 1 expression induced by RNA interference inhibits the bioactivity of hepatocellular carcinoma cell line HCCLM3.

Authors - Wei Jiang