|Positive WB detected in||Jurkat cells, Raji cells, HeLa cells, A431 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, Hela cells|
|Western Blot (WB)||WB : 1:2500-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66026-1-Ig is MCL1 Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||MCL1 fusion protein Ag10609|
|Full Name||myeloid cell leukemia sequence 1 (BCL2-related)|
|Calculated molecular weight||350 aa, 37 kDa|
|Observed molecular weight||37 kDa|
|GenBank accession number||BC107735|
|Gene ID (NCBI)||4170|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.
Downregulation of MicroRNA-125a in Placenta Accreta Spectrum Disorders Contributes Antiapoptosis of Implantation Site Intermediate Trophoblasts by Targeting MCL1.
Reduced ubiquitin-specific protease 9X expression induced by RNA interference inhibits the bioactivity of hepatocellular carcinoma cells.
Mol Med Rep
Sox2 inhibits Wnt-β-catenin signaling and metastatic potency of cisplatin-resistant lung adenocarcinoma cells.
J Bioenerg Biomembr
Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer.
Hsa-miRNA-125b may induce apoptosis of HTR8/SVneo cells by targeting MCL1.
Dig Dis Sci
Reduced high-mobility group box 1 expression induced by RNA interference inhibits the bioactivity of hepatocellular carcinoma cell line HCCLM3.