c-Myc Polyclonal antibody

c-Myc Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10057-1-AP

Synonyms

bHLHe39, c Myc, c-Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 9 publications below

Product Information

10057-1-AP targets c-Myc in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 48 aa, 8 kDa
Observed molecular weight 62-65 kDa, 50 kDa
GenBank accession numberBC000917
Gene symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. This antibody is a rabbit polyclonal antibody raised against recombinant protein of human MYC.The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC [PMID: 12189186]

Publications

SpeciesApplicationTitle
humanWB

J Hazard Mater

Proteomic analysis of cell proliferation in a human hepatic cell line (HL-7702) induced by perfluorooctane sulfonate using iTRAQ.

Authors - Ruina Cui
mouseWB

Mol Med Rep

Mouse bone marrow mesenchymal stem cells with distinct p53 statuses display differential characteristics.

Authors - Bo Wang
humanWB

Mol Cell Biochem

PMID: 21197560

Authors - Xu Bin B
WB

Biochem Biophys Res Commun

miR-612 suppresses the stemness of liver cancer via Wnt/β-catenin signaling.

Authors - Jun Tang
humanWB

Mol Biol Rep

PPARγ mediates the effects of WIN55,212-2, an synthetic cannabinoid, on the proliferation and apoptosis of the BEL-7402 hepatocarcinoma cells.

Authors - Hong Yuehui Y
humanWB

PLoS One

Wogonin Has Multiple Anti-Cancer Effects by Regulating c-Myc/SKP2/Fbw7α and HDAC1/HDAC2 Pathways and Inducing Apoptosis in Human Lung Adenocarcinoma Cell Line A549.

Authors - Chen Xin-Mei XM