SMMHC Polyclonal antibody
SMMHC Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 21404-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse uterus tissue |
| Positive IHC detected in | human colon tissue, human skeletal muscle tissue, rat bladder tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 21404-1-AP is SMMHC Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMMHC fusion protein Ag16114 |
| Full Name | myosin, heavy chain 11, smooth muscle |
| Calculated molecular weight | 1979 aa, 228 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | BC104906 |
| Gene symbol | MYH11 |
| Gene ID (NCBI) | 4629 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMMHC antibody 21404-1-AP | Download protocol |
| IHC protocol for SMMHC antibody 21404-1-AP | Download protocol |
| IF protocol for SMMHC antibody 21404-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Cell Res Autophagy is involved in the differentiation of epicardial progenitor cells into vascular smooth muscle cells in mice. | ||
Nat Commun Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2. | ||
Acta Physiol (Oxf) Prostacyclin facilitates vascular smooth muscle cell phenotypic transformation via activating TP receptors when IP receptors are deficient. |
