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SMMHC Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 21404-1-AP

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

SMMHC Antibody WB mouse uterus tissue 21404-1-AP

mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21404-1-AP(SMMHC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

SMMHC Antibody IHC human skeletal muscle tissue 21404-1-AP

Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21404-1-AP(SMMHC antibody) at dilution of 1:50 (under 10x lens)

SMMHC Antibody IHC human skeletal muscle tissue 21404-1-AP

Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21404-1-AP(SMMHC antibody) at dilution of 1:50 (under 40x lens)

SMMHC Antibody IHC human colon tissue 21404-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21404-1-AP( SMMHC antibody at dilution of 1:200 (under 10x lens).

SMMHC Antibody IHC human colon tissue 21404-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21404-1-AP( SMMHC antibody at dilution of 1:200 (under 40x lens).

SMMHC Antibody IF mouse heart tissue 21404-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 21404-1-AP(SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse uterus tissue, mouse colon tissue
Positive IHC detected in:
human colon tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse heart tissue
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SMMHC fusion protein Ag16114
Full name:
myosin, heavy chain 11, smooth muscle
Calculated molecular weight:
1979aa,228 kDa
Observed molecular weight:
200 kDa
GenBank accession number:
BC104906
Gene ID (NCBI):
4629
Gene symbol
MYH11
Synonyms
MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain

Background

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.


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