SMMHC Polyclonal antibody

SMMHC Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21404-1-AP

Synonyms

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain



Tested Applications

Positive WB detected inmouse uterus tissue, mouse colon tissue
Positive IHC detected inhuman colon tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21404-1-AP is SMMHC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMMHC fusion protein Ag16114
Full Name myosin, heavy chain 11, smooth muscle
Calculated molecular weight 1979 aa, 228 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC104906
Gene symbol MYH11
Gene ID (NCBI) 4629
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

Protocols

Product Specific Protocols
WB protocol for SMMHC antibody 21404-1-APDownload protocol
IHC protocol for SMMHC antibody 21404-1-APDownload protocol
IF protocol for SMMHC antibody 21404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Exp Cell Res

Autophagy is involved in the differentiation of epicardial progenitor cells into vascular smooth muscle cells in mice.

Authors - Yajie Liu
humanWB

Nat Commun

Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2.

Authors - Cheng Wang
mouseWB

Am J Physiol Renal Physiol

PMID: 31066574

Authors - Nao Iguchi
ratWB

FASEB J

CTRP13 attenuates vascular calcification by regulating Runx2.

Authors - Yongxia Li
mouseWB

Sci Adv

Regulated lysosomal exocytosis mediates cancer progression.

Authors - Eda Machado
ratWB

Biomed Pharmacother

Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ.

Authors - Ji-Ru Zhang