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CoraLite® Plus 488-conjugated NSE Monoclonal antibody

NSE Monoclonal Antibody for IF, FC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6F8G3

Cat no : CL488-66150

Synonyms

ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated NSE Monoclonal antib (CL488-66150)

    IF Staining of mouse brain using CL488-66150

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66150 (NSE antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated NSE Monoclonal antib (CL488-66150)

    IF Staining of mouse brain using CL488-66150

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66150 (NSE antibody) at dilution of 1:50.

  • Flow cytometry (FC) experiment of neuronal cells derived from human dental pulp stem using CoraLite® Plus 488-conjugated NSE Monoclonal antib (CL488-66150)

    FC experiment of neuronal cells derived from human dental pulp stem using CL488-66150

    Neuronal cells derived from human dental pulp stem cells were fixed in 70% ethanol and blocked in 0.1% BSA in Staining Buffer, then stained with CoraLite® Plus 488-conjugated NSE Monoclonal Antibody (CL488-66150) (red) or unstained (blue). Image credit: @stemcell_art on Instagram

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated NSE Monoclonal antib (CL488-66150)

    FC experiment of SH-SY5Y using CL488-66150

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NSE (CL488-66150, Clone:6F8G3) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inneuronal cells derived from human dental pulp stem cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66150 targets NSE in IF, FC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NSE fusion protein Ag19106
Full Name enolase 2 (gamma, neuronal)
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC002745
Gene Symbol ENO2
Gene ID (NCBI) 2026
RRIDAB_2883272
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. And this antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NSE antibody CL488-66150Download protocol
Standard Protocols
Click here to view our Standard Protocols