PROS1 Polyclonal antibody

PROS1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16910-1-AP

Synonyms

PROS, PROS1, protein S (alpha), PS21, PS22, PS23, PS24, PS25, PSA, Vitamin K dependent protein S



Tested Applications

Positive WB detected inhuman placenta tissue, mouse placenta tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16910-1-AP targets PROS1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, Plasmodium falciparum
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PROS1 fusion protein Ag10539
Full Name protein S (alpha)
Calculated molecular weight 75 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC015801
Gene symbol PROS1
Gene ID (NCBI) 5627
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

Protocols

Product Specific Protocols
WB protocol for PROS1 antibody 16910-1-APDownload protocol
IHC protocol for PROS1 antibody 16910-1-APDownload protocol
IF protocol for PROS1 antibody 16910-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Med Rep

Hereditary protein S deficiency leads to ischemic stroke.

Authors - Zhao-Hui Wang
Plasmodium falciparumWB,IF

Malar J

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Authors - Takahiro Tougan
mouseWB,IHC

Am J Physiol Heart Circ Physiol

Upregulation of anticoagulant proteins, protein S and tissue factor pathway inhibitor, in the mouse myocardium with cardio-specific TNF-α overexpression.

Authors - Higuchi Yoshihiro Y
humanWB,IF

Am J Hematol

Molecular basis of protein S deficiency in China.

Authors - Tang Liang L
mouseWB

Front Cell Neurosci

Proteomic and Metabolomic Analyses of Vanishing White Matter Mouse Astrocytes Reveal Deregulation of ER Functions.

Authors - Lisanne E Wisse
humanWB

Mol Cell Proteomics

Multisample Mass Spectrometry-Based Approach for Discovering Injury Markers in Chronic Kidney Disease.

Authors - Ji Eun Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hongxue (Verified Customer) (01-25-2021)

Nice band for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver
PROS1 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:16910-1-AP)