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  • KD/KO Validated

S100A6 Polyclonal antibody

S100A6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10245-1-AP

Synonyms

2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6



Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10245-1-AP targets S100A6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A6 fusion protein Ag0385
Full Name S100 calcium binding protein A6
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC001431
Gene symbol S100A6
Gene ID (NCBI) 6277
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.

Protocols

Product Specific Protocols
WB protocol for S100A6 antibody 10245-1-APDownload protocol
IHC protocol for S100A6 antibody 10245-1-APDownload protocol
IF protocol for S100A6 antibody 10245-1-APDownload protocol
IP protocol for S100A6 antibody 10245-1-APDownload protocol
FC protocol for S100A6 antibody 10245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Cancer

ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins.

Authors - Qais Al-Ismaeel
  • KD Validated
ratWB

J Toxicol Environ Health A

Radon-induced proteomic profile of lung tissue in rats.

Authors - Xu Nai-Yu NY
ratWB,IHC

J Toxicol Environ Health A

Proteomic analysis of lung tissue of rats exposed to cigarette smoke and radon.

Authors - Xu Nai-Yu NY
humanWB

Dig Dis Sci

S100A6 as a Potential Serum Prognostic Biomarker and Therapeutic Target in Gastric Cancer.

Authors - Jianli Zhang
  • KD Validated
mouseWB

Mol Reprod Dev

Protein profiling the differences between diabetic and normal mouse cumulus cells.

Authors - Guang-Jian Jiang
monkeyWB

FEBS Lett

S100 proteins regulate the interaction of Hsp90 with Cyclophilin 40 and FKBP52 through their tetratricopeptide repeats.

Authors - Shimamoto Seiko S