- Featured Product
- KD/KO Validated
S100A6 Polyclonal antibody
S100A6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10245-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human pancreas cancer tissue, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
10245-1-AP targets S100A6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | S100A6 fusion protein Ag0385 |
| Full Name | S100 calcium binding protein A6 |
| Calculated molecular weight | 10 kDa |
| Observed molecular weight | 10 kDa |
| GenBank accession number | BC001431 |
| Gene symbol | S100A6 |
| Gene ID (NCBI) | 6277 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for S100A6 antibody 10245-1-AP | Download protocol |
| IHC protocol for S100A6 antibody 10245-1-AP | Download protocol |
| IF protocol for S100A6 antibody 10245-1-AP | Download protocol |
| IP protocol for S100A6 antibody 10245-1-AP | Download protocol |
| FC protocol for S100A6 antibody 10245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Cancer ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins.
| ||
J Toxicol Environ Health A Proteomic analysis of lung tissue of rats exposed to cigarette smoke and radon. | ||
Dig Dis Sci S100A6 as a Potential Serum Prognostic Biomarker and Therapeutic Target in Gastric Cancer.
| ||
Mol Reprod Dev Protein profiling the differences between diabetic and normal mouse cumulus cells. | ||
FEBS Lett S100 proteins regulate the interaction of Hsp90 with Cyclophilin 40 and FKBP52 through their tetratricopeptide repeats. |
