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S100A6 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 10245-1-AP

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Con: 96 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

S100A6 Antibody WB HeLa cells 10245-1-AP

WB result of S100A6 antibody (10245-1-AP, 1:30000) with si-Control and si-S100A6 transfected HeLa cells.

S100A6 Antibody WB HeLa cells 10245-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10245-1-AP(S100A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

S100A6 Antibody IHC human pancreas cancer tissue 10245-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP( S100A6 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

S100A6 Antibody IHC human pancreas cancer tissue 10245-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP( S100A6 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

S100A6 Antibody IF MCF-7 cells 10245-1-AP

Immunofluorescent analysis of MCF-7 cells, using S100A6 antibody 10245-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

S100A6 Antibody FC MCF-7 cells 10245-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug S100A6 antibody (10245-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

S100A6 Antibody IP A549 cells 10245-1-AP

IP Result of anti-S100A6 (IP:10245-1-AP, 3ug; Detection:10245-1-AP 1:1000) with A549 cells lysate 2800ug.

Species specificity:
human, monkey, mouse, rat
Positive WB detected in:
HeLa cells, A549 cells
Positive IP detected in:
A549 cells
Positive IHC detected in:
human pancreas cancer tissue, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
S100A6 fusion protein Ag0385
Full name:
S100 calcium binding protein A6
Calculated molecular weight:
10 kDa
Observed molecular weight:
10 kDa
GenBank accession number:
BC001431
Gene ID (NCBI):
6277
Gene symbol
S100A6
Synonyms
2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6

Background

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.


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