S100A6 Monoclonal antibody
S100A6 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3D11C8
Cat no : 66098-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
The immunogen of 66098-1-Ig is S100A6 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | S100A6 fusion protein Ag17606 |
| Full Name | S100 calcium binding protein A6 |
| Calculated Molecular Weight | 10 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | BC001431 |
| Gene Symbol | S100A6 |
| Gene ID (NCBI) | 6277 |
| RRID | AB_2881497 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for S100A6 antibody 66098-1-Ig | Download protocol |
| IHC protocol for S100A6 antibody 66098-1-Ig | Download protocol |
| IF protocol for S100A6 antibody 66098-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Open Med (Wars) S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53. |
