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TAOK3 Polyclonal antibody

TAOK3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10158-2-AP

Synonyms

DPK, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using TAOK3 Polyclonal antibody (10158-2-AP)

    WB analysis of human liver using 10158-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TAOK3 Polyclonal antibody (10158-2-AP)

    WB analysis of HeLa using 10158-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TAOK3 Polyclonal antibody (10158-2-AP)

    WB analysis of HepG2 using 10158-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using TAOK3 Polyclonal antibody (10158-2-AP)

    WB analysis of human brain using 10158-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TAOK3 Polyclonal antibody (10158-2-AP)

    IP experiment of HepG2 using 10158-2-AP

    IP result of anti-TAOK3 (IP:10158-2-AP, 4ug; Detection:10158-2-AP 1:700) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using TAOK3 Polyclonal antibody (10158-2-AP)

    IHC staining of human liver using 10158-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TAOK3 Polyclonal antibody (10158-2-AP)

    IHC staining of human liver using 10158-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TAOK3 Polyclonal antibody (10158-2-AP)

    IHC staining of human liver using 10158-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TAOK3 Polyclonal antibody (10158-2-AP)

    IHC staining of human liver using 10158-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman liver tissue, HeLa cells, HepG2 cells, human brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10158-2-AP targets TAOK3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAOK3 fusion protein Ag0211
Full Name TAO kinase 3
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC002756
Gene Symbol TAOK3
Gene ID (NCBI) 51347
RRIDAB_2255688
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).

Protocols

Product Specific Protocols
WB protocol for TAOK3 antibody 10158-2-APDownload protocol
IHC protocol for TAOK3 antibody 10158-2-APDownload protocol
IP protocol for TAOK3 antibody 10158-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Commun Signal

Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway.

Authors - Tsung-Ching Lai
  • KD Validated
View Article
mouseIP

Sci Signal

The induction of SHP-1 degradation by TAOK3 ensures the responsiveness of T cells to TCR stimulation

Authors - Alexandre Poirier
View Article