HRP-conjugated alpha Tubulin Antibody

alpha Tubulin Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

HRP Fluorescent Dye

Cat no : HRP-66031

Synonyms

Alpha tubulin ubiquitous, alpha tubulin,a tubulin, HRP Tubulin Alpha, K ALPHA 1, TUBA1B, tubulin, Tubulin Alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b



Tested Applications

Positive WB detected inHeLa cells, Recombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 22 publications below

Product Information

HRP-66031 targets HRP-conjugated alpha Tubulin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
Observed molecular weight 52 kDa
GenBank accession number BC009314
Gene symbol TUBA1B
Gene ID (NCBI) 10376
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the function of alpha-tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerizes to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport, and are also involved in cell division.

 

Where is alpha-tubulin expressed?

Alpha-tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.

 

What are the post-translational modifications of alpha-tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerized microtubule. For example, the first modification to be identified was detyrosination,1 as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyrosination along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3 Polyglutamylation also occurs at several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 

  1. Gundersen, G. G., Khawaja, S. &  Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a  mechanism for subcellular differentiation of microtubules. J. Cell  Biol. 105, 251–64 (1987).

  2. Galjart, N. Plus-End-Tracking Proteins  and Their Interactions at Microtubule Ends. Curr. Biol. 20,  R528–R537 (2010).

  3. Jiang, K. & Akhmanova, A.  Microtubule tip-interacting proteins: a view from both ends. Curr.  Opin. Cell Biol. 23, 94–101 (2011).


Publications

SpeciesApplicationTitle
WB

Oncogene

KLF9-dependent ROS regulate melanoma progression in stage-specific manner.

Authors - Archis Bagati
WB

Dev Comp Immunol

Bombyx mori cypovirus encoded small peptide inhibits viral multiplication.

Authors - Xiaolong Hu
humanWB

Cell Death Dis

Targeting KPNB1 overcomes TRAIL resistance by regulating DR5, Mcl-1 and FLIP in glioblastoma cells.

Authors - Zhi-Chuan Zhu
humanWB

Sci Rep

miR-21-mediated regulation of 15-hydroxyprostaglandin dehydrogenase in colon cancer.

Authors - Nicholas J Monteleone
humanWB

Cell Death Dis

XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis.

Authors - Zhi-Chuan Zhu
humanWB

Oncogene

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Authors - Zhi-Chuan Zhu