HRP-conjugated Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB

Conjugate

HRP Fluorescent Dye

CloneNo.

1E4C11

Cat no : HRP-66031

Synonyms

Alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, HRP Tubulin Alpha, K ALPHA 1, TUBA1B, tubulin, Tubulin Alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin



Tested Applications

Positive WB detected inHeLa cells, Recombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 71 publications below

Product Information

HRP-66031 targets Alpha Tubulin in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC009314
Gene symbol TUBA1B
Gene ID (NCBI) 10376
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the function of alpha-tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerizes to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport, and are also involved in cell division.

 

Where is alpha-tubulin expressed?

Alpha-tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.

 

What are the post-translational modifications of alpha-tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerized microtubule. For example, the first modification to be identified was detyrosination,1 as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyrosination along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3 Polyglutamylation also occurs at several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 

  1. Gundersen, G. G., Khawaja, S. &  Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a  mechanism for subcellular differentiation of microtubules. J. Cell  Biol. 105, 251-64 (1987).

  2. Galjart, N. Plus-End-Tracking Proteins  and Their Interactions at Microtubule Ends. Curr. Biol. 20,  R528-R537 (2010).

  3. Jiang, K. & Akhmanova, A.  Microtubule tip-interacting proteins: a view from both ends. Curr.  Opin. Cell Biol. 23, 94-101 (2011).


Publications

SpeciesApplicationTitle
humanWB

Cell

Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.

Authors - Rui Yang
humanWB

Cell

Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.

Authors - Shicheng Su
humanWB

Immunity

Human Virus-Derived Small RNAs Can Confer Antiviral Immunity in Mammals.

Authors - Yang Qiu
mouseWB

Immunity

Very-low-density lipoprotein receptor-enhanced lipid metabolism in pancreatic stellate cells promotes pancreatic fibrosis.

Authors - Xuguang Yang
mouseWB

Nat Immunol

Control of CD1d-restricted antigen presentation and inflammation by sphingomyelin.

Authors - Espen Melum
humanWB

Nat Commun

Regulation of local GTP availability controls RAC1 activity and cell invasion

Authors - Anna Bianchi-Smiraglia