Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, mouse pancreas tissue, mouse brain tissue, human kidney tissue, human heart tissue, HeLa cells, K-562 cells, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
19140-1-AP targets VPS11 in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VPS11 fusion protein Ag6227 Predict reactive species |
| Full Name | vacuolar protein sorting 11 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 108 kDa |
| Observed Molecular Weight | 108-112 kDa |
| GenBank Accession Number | BC065563 |
| Gene Symbol | VPS11 |
| Gene ID (NCBI) | 55823 |
| RRID | AB_10642572 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H270 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vesicle-mediated protein sorting plays an important role in segregating intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of the HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and membrane docking/fusion reactions of late endosomes/lysosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS11 antibody 19140-1-AP | Download protocol |
| IP protocol for VPS11 antibody 19140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
J Cell Sci Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.
| ||
J Mol Biol Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B. | ||
J Biol Chem Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity. | ||
J Cell Sci The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.
| ||
