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  • KD/KO Validated

VPS11 Polyclonal antibody

VPS11 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19140-1-AP

Synonyms

END1, hVPS11, PEP5, RING finger protein 108, RNF108, VPS11



Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, human heart tissue, human kidney tissue, mouse brain tissue, mouse pancreas tissue
Positive IP detected inHEK-293 cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19140-1-AP is VPS11 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS11 fusion protein Ag6227
Full Name vacuolar protein sorting 11 homolog (S. cerevisiae)
Calculated molecular weight 108 kDa
Observed molecular weight 108-112 kDa
GenBank accession numberBC065563
Gene symbol VPS11
Gene ID (NCBI) 55823
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for VPS11 antibody 19140-1-APDownload protocol
IF protocol for VPS11 antibody 19140-1-APDownload protocol
IP protocol for VPS11 antibody 19140-1-APDownload protocol
FC protocol for VPS11 antibody 19140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

The second report of a new hypomyelinating disease due to a defect in the VPS11 gene discloses a massive lysosomal involvement.

Authors - Konstanze Hörtnagel
humanWB,IP

Biochem J

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.

Authors - Morag R Hunter
humanIF,WB

J Cell Sci

The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.

Authors - Magdalena Banach-Orłowska
  • KD Validated
humanWB, IF

J Biol Chem

Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.

Authors - Rik van der Kant
humanWB

J Mol Biol

Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.

Authors - Morag R Hunter
humanWB,IF

J Cell Sci

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Authors - van der Kant Rik R
  • KO Validated