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- KD/KO Validated
VPS11 Polyclonal antibody
VPS11 Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 19140-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, HEK-293 cells, human heart tissue, human kidney tissue, mouse brain tissue, mouse pancreas tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 19140-1-AP is VPS11 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VPS11 fusion protein Ag6227 |
| Full Name | vacuolar protein sorting 11 homolog (S. cerevisiae) |
| Calculated molecular weight | 108 kDa |
| Observed molecular weight | 108-112 kDa |
| GenBank accession number | BC065563 |
| Gene symbol | VPS11 |
| Gene ID (NCBI) | 55823 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS11 antibody 19140-1-AP | Download protocol |
| IF protocol for VPS11 antibody 19140-1-AP | Download protocol |
| IP protocol for VPS11 antibody 19140-1-AP | Download protocol |
| FC protocol for VPS11 antibody 19140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Inherit Metab Dis The second report of a new hypomyelinating disease due to a defect in the VPS11 gene discloses a massive lysosomal involvement. | ||
J Cell Sci The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.
| ||
J Biol Chem Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity. | ||
J Mol Biol Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B. | ||
J Cell Sci Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.
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