HEK-293 cells were subjected to SDS PAGE followed by western blot with 19140-1-AP(VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

human brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP(VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

human heart tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP(VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

human kidney tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP(VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP(VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IP Result of anti-VPS11 (IP:19140-1-AP, 4ug; Detection:19140-1-AP 1:500) with HEK-293 cells lysate 1480ug.

Immunofluorescent analysis of HepG2 cells, using VPS11 antibody 19140-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

1X10^6 HepG2 cells were stained with 0.2ug VPS11 antibody (19140-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.