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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human Wnt3A

Activity

Typically ≤ 20 ng/mL EC50

Species Reactivity

human

Purity

>90%

Cat no : HZ-1296

Synonyms

Protein Wnt 3a, WNT3A



Technical Specifications

GeneID 89780
Species human
Expression HEK293
Activity Typically ≤ 20 ng/mL EC50
Purity >90%
Molecular Mass 38-42 kDa
Formulation 1x PBS pH 7.4 + 500mM NaCl +1% CHAPS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Wnt3A is a member of the Wnt family that consists of structurally linked genes that encode secreted signalling proteins. This protein is associated with oncogenesis and also regulation of cell fate and patterning during embryogenesis. It plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnt are essential for their efficient secretion and biological functions (PMID: 8244403; 11834740)

Synonyms

Protein Wnt 3a, WNT3A

Publications

SpeciesTitle
human

Sci Signal

Intercellular transmission of the unfolded protein response promotes survival and drug resistance in cancer cells.

Authors - Jeffrey J Rodvold