CoraLite®594-conjugated transgelin/SM22 Monoclonal antibody

transgelin/SM22 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

IF, ELISA

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A10C2

Cat no : CL594-60213

Synonyms

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, WS3 10



Tested Applications

Positive IF detected inmouse heart tissue
For other applications, we recommend the unconjugated version of this antibody, 60213-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-60213 is transgelin/SM22 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 18+22 kDa
GenBank accession numberBC004927
Gene symbol TAGLN
Gene ID (NCBI) 6876
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.

Protocols

Product Specific Protocols
IF protocol for CL594-conjugated transgelin/SM22 antibody CL594-60213Download protocol
Standard Protocols
Click here to view our Standard Protocols