transgelin/SM22 Monoclonal antibody

transgelin/SM22 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, rat, pig, mouse, mouse







Cat no : 60213-1-Ig


22 kDa actin binding protein, SM22, SM22 alpha, SM22a, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, transgelin/SM22, transgelin/SM22 alpha

Tested Applications

Positive WB detected inpig colon tissue, human colon tissue, pig stomach tissue, human stomach tissue, rat colon tissue, rat stomach tissue, rabbit stomach tissue, mouse colon tissue
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60213-1-Ig targets transgelin/SM22 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, pig, mouse, mouse samples.

Tested Reactivity human, rat, pig, mouse, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 18+22 kDa
GenBank accession numberBC004927
Gene symbol TAGLN
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.


Product Specific Protocols
WB protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
IHC protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
IF protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ana Luísa (Verified Customer) (07-25-2022)

No permeabilization. Blocking with 5%FBS and 2%BSA. Some background

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Intervertebral disc cells