transgelin/SM22 Monoclonal antibody
transgelin/SM22 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
human, rat, pig, mouse
WB, IHC, IF, FC, ELISA
Cat no : 60213-1-Ig
Validation Data Gallery
|Positive WB detected in||human colon tissue, human stomach tissue, pig stomach tissue, rat colon tissue, rat stomach tissue|
|Positive IHC detected in||human colon tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 5 publications below|
|IF||See 5 publications below|
|FC||See 1 publications below|
The immunogen of 60213-1-Ig is transgelin/SM22 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat, pig, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||transgelin/SM22 fusion protein Ag0764|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||18+22 kDa|
|GenBank accession number||BC004927|
|Gene ID (NCBI)||6876|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.
|Product Specific Protocols|
|WB protocol for transgelin/SM22 antibody 60213-1-Ig||Download protocol|
|IHC protocol for transgelin/SM22 antibody 60213-1-Ig||Download protocol|
|IF protocol for transgelin/SM22 antibody 60213-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
LDHA mediated degradation of extracellular matrix is a potential target for the treatment of aortic dissection.
Specific role of RhoC in tumor invasion and metastasis.
Front Cell Dev Biol
Platelet-Derived Microvesicles Promote VSMC Dedifferentiation After Intimal Injury via Src/Lamtor1/mTORC1 Signaling
Biochim Biophys Acta Mol Basis Dis
Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ.
Front Cardiovasc Med
Insulin Resistance Promotes the Formation of Aortic Dissection by Inducing the Phenotypic Switch of Vascular Smooth Muscle Cells.
Asian J Androl
The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes model via mitochondrial protection.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ana Luísa (Verified Customer) (07-25-2022)
No permeabilization. Blocking with 5%FBS and 2%BSA. Some background