|Positive WB detected in||human colon tissue, human stomach tissue, pig stomach tissue, rat colon tissue, rat stomach tissue|
|Positive IHC detected in||human colon tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60213-1-Ig is transgelin/SM22 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat, pig, mouse|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||transgelin/SM22 fusion protein Ag0764|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||18+22 kDa|
|GenBank accession number||BC004927|
|Gene ID (NCBI)||6876|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.
Characterization of Carotid Smooth Muscle Cells during Phenotypic Transition.
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