transgelin/SM22 Monoclonal antibody

transgelin/SM22 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10C2

Cat no : 60213-1-Ig

Synonyms

22 kDa actin binding protein, SM22, SM22 alpha, SM22a, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, transgelin/SM22, transgelin/SM22 alpha



Tested Applications

Positive WB detected inpig colon tissue, human colon tissue, pig stomach tissue, human stomach tissue, rat colon tissue, rat stomach tissue, rabbit stomach tissue, mouse colon tissue
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60213-1-Ig targets transgelin/SM22 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, pig, mouse, mouse samples.

Tested Reactivity human, rat, pig, mouse, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 18+22 kDa
GenBank accession numberBC004927
Gene symbol TAGLN
Gene ID (NCBI) 6876
RRIDAB_11043177
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.

Protocols

Product Specific Protocols
WB protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
IHC protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
IF protocol for transgelin/SM22 antibody 60213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Pharmacol Res

LDHA mediated degradation of extracellular matrix is a potential target for the treatment of aortic dissection.

Authors - Xiaohui Wu
humanWB

Oncotarget

Specific role of RhoC in tumor invasion and metastasis.

Authors - Sarah Lang
mouseWB

Front Cell Dev Biol

Platelet-Derived Microvesicles Promote VSMC Dedifferentiation After Intimal Injury via Src/Lamtor1/mTORC1 Signaling

Authors - Ji-Ting Liu
ratIF

Biochim Biophys Acta Mol Basis Dis

Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ.

Authors - Bao-Ying Luo
human,mouseWB,IF

Front Cardiovasc Med

Insulin Resistance Promotes the Formation of Aortic Dissection by Inducing the Phenotypic Switch of Vascular Smooth Muscle Cells.

Authors - Hui Zheng
ratIF,WB

Asian J Androl

The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes model via mitochondrial protection.

Authors - Xiao-Feng Yue

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ana Luísa (Verified Customer) (07-25-2022)

No permeabilization. Blocking with 5%FBS and 2%BSA. Some background

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Intervertebral disc cells