transgelin/SM22 Monoclonal antibody
transgelin/SM22 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig, mouse
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2A10C2
Cat no : 60213-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human colon tissue, human stomach tissue, pig stomach tissue, rat colon tissue, rat stomach tissue |
| Positive IHC detected in | human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 5 publications below |
| IF | See 5 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 60213-1-Ig is transgelin/SM22 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, rat, pig, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | transgelin/SM22 fusion protein Ag0764 |
| Full Name | transgelin |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 18+22 kDa |
| GenBank accession number | BC004927 |
| Gene symbol | TAGLN |
| Gene ID (NCBI) | 6876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for transgelin/SM22 antibody 60213-1-Ig | Download protocol |
| IHC protocol for transgelin/SM22 antibody 60213-1-Ig | Download protocol |
| IF protocol for transgelin/SM22 antibody 60213-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pharmacol Res LDHA mediated degradation of extracellular matrix is a potential target for the treatment of aortic dissection. | ||
Front Cell Dev Biol Platelet-Derived Microvesicles Promote VSMC Dedifferentiation After Intimal Injury via Src/Lamtor1/mTORC1 Signaling | ||
Biochim Biophys Acta Mol Basis Dis Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ. | ||
Front Cardiovasc Med Insulin Resistance Promotes the Formation of Aortic Dissection by Inducing the Phenotypic Switch of Vascular Smooth Muscle Cells. | ||
Asian J Androl The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes model via mitochondrial protection. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ana Luísa (Verified Customer) (07-25-2022) | No permeabilization. Blocking with 5%FBS and 2%BSA. Some background
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