MAGEA3 Monoklonaler Antikörper

MAGEA3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1A9F3

Kat-Nr. : 60054-1-Ig

Synonyme

Antigen MZ2 D, Cancer/testis antigen 1.3, CT1.3, HIP8, HYPD, MAGE 3 antigen, MAGE3, MAGEA3, MAGE-A3, MAGEA6, melanoma antigen family A, 3, Melanoma associated antigen 3

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  WB analysis of mouse brain using 60054-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 60054-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 60054-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60054-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 60054-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 60054-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, fetales humanes Hirngewebe, humanes Hodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

60054-1-Ig bindet in WB, IHC, ELISA MAGEA3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAGEA3 fusion protein Ag0265
• Vollständiger Name: melanoma antigen family A, 3
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC000340
• Gene symbol: MAGEA3
• Gene ID (NCBI): 4102
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGEA3 is a member of the MAGEA family, , which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.

Publikationen

Species	Application	Title
human	WB	PLoS One; MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation.; Authors - Aaron Chen
human	WB	J Transl Med; Immunization with a multi-antigen targeted DNA vaccine eliminates chemoresistant pancreatic cancer by disrupting tumor-stromal cell crosstalk; Authors - Hongquan Qin