WFS1 Polyklonaler Antikörper

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• KD/KO Validated

WFS1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, coIP, ELISA

Konjugation

Unkonjugiert

Publikationen

70

Rezensionen (1)

Kat-Nr. : 11558-1-AP

Synonyme

WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN

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Galerie der Validierungsdaten

• 

  WB analysis of SH-SY5Y using 11558-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11558-1-AP (WFS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11558-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11558-1-AP (WFS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 11558-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11558-1-AP (WFS1 antibody) at dilution of 1:400 (under 40x lens)..

• 

  IF Staining of mouse brain using 11558-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11558-1-AP (WFS1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66564-1-Ig (Tbr1 antibody) and and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat brain using 11558-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using WFS1 antibody (11558-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 11558-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11558-1-AP (WFS1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 17 publications below
• IF: See 35 publications below
• CoIP: See 1 publications below

Produktinformation

11558-1-AP bindet in WB, IHC, IF, coIP, ELISA WFS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WFS1 fusion protein Ag2114
• Vollständiger Name: Wolfram syndrome 1 (wolframin)
• Berechnetes Molekulargewicht: 890 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC030130
• Gene symbol: WFS1
• Gene ID (NCBI): 7466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

Publikationen

Species	Application	Title
mouse	IF	Science; Monosomes actively translate synaptic mRNAs in neuronal processes.; Authors - Anne Biever
mouse	IF	Cell; A Hippocampus-Accumbens Tripartite Neuronal Motif Guides Appetitive Memory in Space.; Authors - Stéphanie Trouche
mouse	IF	Nature; The hippocampal CA2 region is essential for social memory.; Authors - Frederick L Hitti
mouse	IF	Science; Island cells control temporal association memory.; Authors - Takashi Kitamura
mouse	IF	Cell; A Map-like Micro-Organization of Grid Cells in the Medial Entorhinal Cortex.; Authors - Yi Gu
mouse	IF	Cell; Distinct Neural Circuits for the Formation and Retrieval of Episodic Memories.; Authors - Dheeraj S Roy

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (05-29-2020)

SDS-PAGE: 15 ug/ul RIPA lysate of whole brain tissue or mitochondrial fraction. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000Lines of WB: 1 – protein ladder, 2 – whole brain tissue lysate, 3 – mitochondrial fraction lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Whole brain tissue (human), mitochondrial fraction from brain tissue (human).

WFS1 Antibody Western Blot validation (1:1000 dilution) in Whole brain tissue (human), mitochondrial fraction from brain tissue (human). (Cat no:11558-1-AP)

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