Anticorps Monoclonal anti-MAGEA3

MAGEA3 Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1A9F3

N° de cat : 60054-1-Ig

Synonymes

Antigen MZ2 D, Cancer/testis antigen 1.3, CT1.3, HIP8, HYPD, MAGE 3 antigen, MAGE3, MAGEA3, MAGE-A3, MAGEA6, melanoma antigen family A, 3, Melanoma associated antigen 3

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 60054-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60054-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60054-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60054-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 60054-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60054-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 60054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain fœtal, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'œsophage humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

60054-1-Ig cible MAGEA3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAGEA3 Protéine recombinante Ag0265
• Nom complet: melanoma antigen family A, 3
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 35-55 kDa
• Numéro d’acquisition GenBank: BC000340
• Symbole du gène: MAGEA3
• Identification du gène (NCBI): 4102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	PLoS One; MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation.; Authors - Aaron Chen
human	WB	J Transl Med; Immunization with a multi-antigen targeted DNA vaccine eliminates chemoresistant pancreatic cancer by disrupting tumor-stromal cell crosstalk; Authors - Hongquan Qin

Produits associés

MAGEA1 Polyclonal antibody

55105-1-AP

MAGEA1 Polyclonal antibody

12233-1-AP

MAGEA4 Polyclonal antibody

55109-1-AP

MAGEA4 Polyclonal antibody

12508-1-AP

KAP1 Polyclonal antibody

15202-1-AP

P53 Polyclonal antibody

10442-1-AP

UBE2E2 Polyclonal antibody

11844-1-AP

UBE2E3 Polyclonal antibody

15488-1-AP