Anticorps Polyclonal de lapin anti-TGF Beta 1

• Phare
• Validé par KD/KO

TGF Beta 1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

529

Avis (7)

N° de cat : 21898-1-AP

Synonymes

CED, DPD1, LAP, Mouse Transforming Growth Factor β1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, TGF-beta 1, tgfbeta1, tgf-beta1, TGFβ, TGF-β, TGFβ1, TGF-β1

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Galerie de données de validation

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  WB analysis using 21898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21898-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21898-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21898-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 21898-1-AP

  IP result of anti-TGF Beta 1(IP:21898-1-AP, 4ug; Detection:21898-1-AP 1:6000) with mouse skin tissue lysate 640 ug.

• 

  IF Staining of HEK-293 using 21898-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HEK-293 using 21898-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human TGF Beta 1 (21898-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, cellules A549, cellules HeLa, cellules K-562, tissu cutané de rat
• Résultats positifs en IP: tissu cutané de souris,
• Résultats positifs en IF: cellules HEK-293,
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 416 publications below
• IHC: See 121 publications below
• IF: See 53 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21898-1-AP cible TGF Beta 1 dans les applications de WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, Lapin, poisson-zèbre, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGF Beta 1 Protéine recombinante Ag13591
• Nom complet: transforming growth factor, beta 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC000125
• Symbole du gène: TGFB1
• Identification du gène (NCBI): 7040
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.

Publications

Species	Application	Title
	IHC	Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
human	WB	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
	WB,IF	Acta Pharm Sin B; Fucoidan-functionalized activated platelet-hitchhiking micelles simultaneously track tumor cells and remodel the immunosuppressive microenvironment for efficient metastatic cancer treatment.; Authors - Rong Guo
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
		Autophagy; Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration.; Authors - Paola Rusmini

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (08-15-2023)

This antibody works well for labeling TGF beta 1 on mouse kidney tissue section. Very reliable antibody giving reproducible results.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse Kidney

FH

Celina (Verified Customer) (07-31-2023)

antibody worked well for IF of human cardiac ventricular fibroblasts

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human cardiac ventricular fibroblasts

FH

Udesh (Verified Customer) (12-06-2022)

The antibody worked well in Western Blot and detected bands for mature TGF-B1 as well as LAP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

TGF Beta 1 Antibody Western Blot validation (1:1000 dilution) in (Cat no:21898-1-AP)

FH

Yasuyo (Verified Customer) (05-24-2022)

Works great!!!

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: lung

TGF Beta 1 Antibody Immunohistochemistry validation (1:250 dilution) in lung (Cat no:21898-1-AP)

FH

P (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: fibroblast

TGF Beta 1 Antibody Western Blot validation ( dilution) in fibroblast (Cat no:21898-1-AP)

FH

Iram (Verified Customer) (09-04-2020)

Very clean bands for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Human colon cancer

FH

Qingyuan (Verified Customer) (11-05-2019)

The antibody clearly shows one band with the right molecular weight size.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse spinal cord

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C