Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | PHA treated human PBMCs, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-81746-2 targets TGF Beta 1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13591 Product name: Recombinant human TGFB1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 14-258 aa of BC000125 Sequence: PLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFL Predict reactive species |
| Full Name | transforming growth factor, beta 1 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC000125 |
| Gene Symbol | TGFB1 |
| Gene ID (NCBI) | 7040 |
| RRID | AB_3749556 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01137 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE TGF Beta 1 antibody PE-81746-2 | Download protocol |
| IF protocol for PE TGF Beta 1 antibody PE-81746-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One user (Courtney F.) rated this PE-conjugated antibody 5 stars for immunofluorescence on RPTEC cells at a 1:125 dilution. They reported strong target expression and successful imaging using a TRITC filter on their microscope, indicating the conjugate performed well out of the box for their IF workflow.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Courtney (Verified Customer) (06-30-2026) | PE conjugated antibody had great expression and I was able to image using the TRITC filter on our microscope.
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