Filter: AllWBSandwich ELISA sample testSandwich ELISACytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 87058-2-RR (same clone as 87058-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87058-2-RR (PDLIM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87058-2-PBS in a different storage buffer formulation.
WB analysis of human heart using 87058-2-RR (same clone as 87058-2-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 87058-2-RR (PDLIM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87058-2-PBS in a different storage buffer formulation.
WB analysis using 87058-2-RR (same clone as 87058-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87058-2-RR (PDLIM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87058-2-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP02852-1
The mean PDLIM3 concentration was determined to be 321.00 ng/mL in mouse skeletal muscle extract based on a 4.10 mg/mL extract load, 29.50 ng/mL in mouse heart extract based on a 5.40 mg/mL extract load, 6.40 ng/mL in mouse liver extract based on a 8.40 mg/mL extract load and 14.40 ng/mL in mouse kidney extract based on a 8.4 mg/mL extract load.
Sandwich ELISA standard curve of MP02852-1
Sandwich ELISA standard curve of MP02852-1, Human PDLIM3 Recombinant Matched Antibody Pair - PBS only. 87058-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3934. 87058-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.
Cytometric bead array sample test of MP02852-1
The mean PDLIM3 concentration was determined to be 495.6 ng/mL in mouse spleen tissue extract based on a 6.4 mg/mL extract load.
Cytometric bead array standard curve of MP02852-1
Cytometric bead array standard curve of MP02852-1, PDLIM3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87058-2-PBS. Detection antibody: 87058-1-PBS. Standard: Ag3934. Range: 0.156-20 ng/mL.
Affinity of 87058-2-RR
Biolayer interferometry (BLl) kinetic assays of 87058-2-RR against Human MCM6 were performed. The affinity constant is 0.242 nM.

PDLIM3 Recombinant monoclonal antibody, PBS Only (Capture)

Name: PDLIM3 Recombinant monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 87058-2-PBS

PDLIM3 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 87058-2-PBS

Clone No.252308B8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Actinin associated LIM protein, Actinin-associated LIM protein, ALP, Alpha-actinin-2-associated LIM protein, PDZ and LIM domain 3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87058-2-PBS targets PDLIM3 as part of a matched antibody pair:

MP02852-1: 87058-2-PBS capture and 87058-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag3934 Product name: Recombinant human PDLIM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC027870 Sequence: MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQNEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTKVHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTKPPEGYDTVTLYPKA Predict reactive species
Full Name	PDZ and LIM domain 3
Calculated Molecular Weight	316 aa, 34 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC027870
Gene Symbol	PDLIM3
Gene ID (NCBI)	27295
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q53GG5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PDLIM3, also known as ALP, contains a PDZ domain and a LIM domain. PDLIM3 binds the spectrin-like repeats of alpha-actinin-2 and colocalizes with alpha-actinin-2 at the Z lines of skeletal muscle.