Recombinant Human Fibrinogen Alpha Chain protein (rFc Tag)
Species
Human
Purity
>95 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4643
Validation Data Gallery
Product Information
| Purity | >95 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Fibrinogen Alpha Chain protein Lys620-Gln866 (Accession# P02671-1) with a rabbit IgG Fc tag at the N-terminus |
| GeneID | 2243 |
| Accession | P02671-1 |
| PredictedSize | 55.1 kDa |
| SDS-PAGE | 60-65 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia.
References:
Mosesson, M W. Journal of thrombosis and haemostasis : JTH vol. 3,8 (2005): 1894-904.