ABCE1 Polyclonal antibody

ABCE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28548-1-AP

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

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Validation Data Gallery

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  WB analysis using 28548-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28548-1-AP (ABCE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28548-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 28548-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, NCI-H1299 cells
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

28548-1-AP targets ABCE1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCE1 fusion protein Ag29724
• Full Name: ATP-binding cassette, sub-family E (OABP), member 1
• Calculated molecular weight: 599 aa, 67 kDa
• Observed molecular weight: 68 kDa
• GenBank accession number: BC016283
• Gene symbol: ABCE1
• Gene ID (NCBI): 6059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.