Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23082-1-AP

Synonyms

Alpha 1 actin, Alpha actin



Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse colon tissue, human small intestine tissue, human colon tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23082-1-AP is Alpha Actin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actin fusion protein Ag19482
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC009978
Gene symbol ACTC1
Gene ID (NCBI) 70
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Alpha Actin antibody 23082-1-APDownload protocol
IHC protocol for Alpha Actin antibody 23082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Circ Heart Fail

Phenotype and Physiological Significance of the Endocardial Smooth Muscle Cells in Human Failing Hearts.

Authors - Hideshi Okada