Tested Applications
Positive WB detected in | mouse heart tissue |
Positive IHC detected in | human normal colon, human colon tissue, human heart tissue, human skeletal muscle tissue, human small intestine tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
23082-1-AP targets Alpha cardiac muscle actin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen |
CatNo: Ag19482 Product name: Recombinant human ACTC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-50 aa of BC009978 Sequence: MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG Predict reactive species |
Full Name | actin, alpha, cardiac muscle 1 |
Calculated Molecular Weight | 377 aa, 42 kDa |
Observed Molecular Weight | 42-45 kDa |
GenBank Accession Number | BC009978 |
Gene Symbol | ACTC1 |
Gene ID (NCBI) | 70 |
RRID | AB_2879206 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P68032 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Alpha cardiac muscle actin antibody 23082-1-AP | Download protocol |
IHC protocol for Alpha cardiac muscle actin antibody 23082-1-AP | Download protocol |
FC protocol for Alpha cardiac muscle actin antibody 23082-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |