ACVRL1 Polyclonal antibody, PBS Only

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  WB analysis of human placenta using 14745-1-AP (same clone as 14745-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 14745-1-AP (same clone as 14745-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 14745-1-AP (same clone as 14745-1-PBS)

  IP result of anti-ACVRL1 (IP:14745-1-AP, 4ug; Detection:14745-1-AP 1:300) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 14745-1-AP (same clone as 14745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14745-1-AP (ACVRL1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 14745-1-AP (same clone as 14745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 14745-1-AP (same clone as 14745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 14745-1-AP (same clone as 14745-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug ACVRL1 Polyclonal antibody (14745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14745-1-PBS in a different storage buffer formulation.

ACVRL1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Cat No. 14745-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

ALK-1, ALK1, ALK 1, ACVRLK1, Activin receptor-like kinase 1

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Product Information

14745-1-PBS targets ACVRL1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6449

  Product name: Recombinant human ACVRL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 142-503 aa of BC042637

  Sequence: HVRRRQEKQRGLHSELGESSLILKASEQGDSMLGDLLDSDCTTGSGSGLPFLVQRTVARQVALVECVGKGRYGEVWRGLWHGESVAVKIFSSRDEQSWFRETEIYNTVLLRHDNILGFIASDMTSRNSSTQLWLITHYHEHGSLYDFLQRQTLEPHLALRLAVSAACGLAHLHVEIFGTQGKPAIAHRDFKSRNVLVKSNLQCCIADLGLAVMHSQGSDYLDIGNNPRVGTKRYMAPEVLDEQIRTDCFESYKWTDIWAFGLVLWEIARRTIVNGIVEDYRPPFYDVVPNDPSFEDMKKVVCVDQQTPTIPNRLAADPVLSGLAQMMRECWYPNPSARLTALRIKKTLQKISNSPEKPKVIQ

• Full Name: activin A receptor type II-like 1
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC042637
• Gene Symbol: ACVRL1
• Gene ID (NCBI): 94
• RRID: AB_2222474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P37023
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.