ADRA1A-Specific Polyclonal antibody, PBS Only

• 

  WB analysis using 19777-1-AP (same clone as 19777-1-PBS)

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours without or with blocking peptide (1.4 ug peptide was used to block the binding of 1.0 ug antibody). This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19777-1-AP (same clone as 19777-1-PBS)

  WB result of ADRA1A-Specific antibody (19777-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADRA1A-Specific transfected HeLa cells. This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19777-1-AP (same clone as 19777-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 19777-1-AP (same clone as 19777-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 19777-1-AP (same clone as 19777-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRA1A-Specific Polyclonal antibody (19777-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 19777-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ADRA1A-Specific Polyclonal Antibody for WB, Indirect ELISA

Cat No. 19777-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

ADRA1A, Alpha-1C adrenergic receptor, Alpha-1A adrenoreceptor, Alpha-1A adrenoceptor, Alpha-1A adrenergic receptor

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Product Information

19777-1-PBS targets ADRA1A-Specific in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: adrenergic, alpha-1A-, receptor
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_000680
• Gene Symbol: ADRA1A
• Gene ID (NCBI): 148
• RRID: AB_10643238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35348
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ADRA1A, also named as ADRA1C, belongs to the G-protein coupled receptor 1 family. ADRA1A mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. The antibody is specific to ADRA1A.