AGR2 Monoclonal antibody

AGR2 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b


Human, Pig and More (1)







Cat no : 66768-1-Ig


AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2

Tested Applications

Positive WB detected inpig stomach tissue, T-47D cells, HT-29 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHT-29 cells, human colon cancer tissue
Positive FC detected inHT-29 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66768-1-Ig targets AGR2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AGR2 fusion protein Ag2919
Full Name anterior gradient homolog 2 (Xenopus laevis)
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC015503
Gene Symbol AGR2
Gene ID (NCBI) 10551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.


Product Specific Protocols
WB protocol for AGR2 antibody 66768-1-IgDownload protocol
IHC protocol for AGR2 antibody 66768-1-IgDownload protocol
IF protocol for AGR2 antibody 66768-1-IgDownload protocol
FC protocol for AGR2 antibody 66768-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cancer

LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription.

Authors - Bingqiu Xiu

Front Oncol

QKI-6 Suppresses Cell Proliferation, Migration, and EMT in Non-Small Cell Lung Cancer.

Authors - Haihua Zhang