CoraLite® Plus 488-conjugated AGR2 Monoclonal antibody

AGR2 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A8A8

Cat no : CL488-66768

Synonyms

GOB 4, AGR 2, AG-2, AG2, AG 2

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Validation Data Gallery

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  IF Staining of human colon cancer using CL488-66768

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

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  IF Staining of HT-29 using CL488-66768

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite®®488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: HT-29 cells, human colon cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66768 targets AGR2 in IF/ICC, IF-P applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: AGR2 fusion protein Ag2919
• Full Name: anterior gradient homolog 2 (Xenopus laevis)
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC015503
• Gene Symbol: AGR2
• Gene ID (NCBI): 10551
• RRID: AB_2919366
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.