AP5Z1/SPG48 Monoclonal antibody
AP5Z1/SPG48 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1G1D7
Cat no : 66533-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
66533-1-Ig targets AP5Z1/SPG48 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.
Tested Reactivity | Human, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AP5Z1/SPG48 fusion protein Ag26961 |
Full Name | KIAA0415 |
Calculated molecular weight | 807 aa, 88 kDa |
Observed molecular weight | 88 kDa, 160 kDa |
GenBank accession number | BC037399 |
Gene symbol | KIAA0415 |
Gene ID (NCBI) | 9907 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.
Protocols
Product Specific Protocols | |
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WB protocol for AP5Z1/SPG48 antibody 66533-1-Ig | Download protocol |
IHC protocol for AP5Z1/SPG48 antibody 66533-1-Ig | Download protocol |
IF protocol for AP5Z1/SPG48 antibody 66533-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |