CoraLite®594-conjugated AP5Z1/SPG48 Monoclonal antibody

AP5Z1/SPG48 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G1D7

Cat no : CL594-66533

Synonyms

KIAA0415, SPG48

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Validation Data Gallery

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  IF Staining of HEK-293 using CL594-66533

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 AP5Z1/SPG48 antibody (CL594-66533, Clone: 1G1D7 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66533 targets AP5Z1/SPG48 in IF applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AP5Z1/SPG48 fusion protein Ag26961
• Full Name: KIAA0415
• Calculated Molecular Weight: 807 aa, 88 kDa
• Observed Molecular Weight: 88 kDa, 160 kDa
• GenBank Accession Number: BC037399
• Gene Symbol: KIAA0415
• Gene ID (NCBI): 9907
• RRID: AB_2920003
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.