CoraLite® Plus 488-conjugated AP5Z1/SPG48 Monoclonal antibody

AP5Z1/SPG48 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1D7

Cat no : CL488-66533

Synonyms

AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5

---

Validation Data Gallery

• 

  IF Staining of HEK-293 using CL488-66533

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 AP5Z1/SPG48 antibody (CL488-66533, Clone: 1G1D7 ) at dilution of 1:100.

---

Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66533 targets AP5Z1/SPG48 in IF/ICC applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AP5Z1/SPG48 fusion protein Ag26961
• Full Name: KIAA0415
• Calculated Molecular Weight: 807 aa, 88 kDa
• Observed Molecular Weight: 88 kDa, 160 kDa
• GenBank Accession Number: BC037399
• Gene Symbol: AP5Z1/SPG48
• Gene ID (NCBI): 9907
• RRID: AB_2919340
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.