CoraLite® Plus 488-conjugated ASCT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ASCT2 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20350

Synonyms

AAAT, ASCT2, ATB(0), ATBO, Baboon M7 virus receptor, FLJ31068, M7V1, M7VS1, R16, RDR, RDRC, SLC1A5

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Validation Data Gallery

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  IF Staining of mouse small intestine using CL488-20350

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 ASCT2 antibody (CL488-20350) at dilution of 1:100, Villin antibody (16488-1-AP, Magenta), CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

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  IF Staining of mouse small intestine using CL488-20350

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 ASCT2 antibody (CL488-20350) at dilution of 1:100, Villin antibody (16488-1-AP, Magenta), CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

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  FC experiment of HeLa using CL488-20350

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ASCT2 (CL488-20350) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse small intestine tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-20350 targets ASCT2 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASCT2 fusion protein Ag14182
• Full Name: solute carrier family 1 (neutral amino acid transporter), member 5
• Calculated Molecular Weight: 541 aa, 57 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC000062
• Gene Symbol: SLC1A5
• Gene ID (NCBI): 6510
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.