CoraLite®488-conjugated ATP5O Monoclonal antibody

ATP5O Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1D3B8

Cat no : CL488-66696

Synonyms

ATP5O, ATPO, OSCP



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66696-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66696 targets ATP5O in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5O fusion protein Ag1458
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Calculated molecular weight 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC021233
Gene symbol ATP5O
Gene ID (NCBI) 539
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.

Protocols

Product Specific Protocols
IF protocol for CL488 ATP5O antibody CL488-66696Download protocol
Standard Protocols
Click here to view our Standard Protocols