ATP5O Monoclonal antibody

ATP5O Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D3B8

Cat no : 66696-1-Ig

Synonyms

ATP5O, ATPO, OSCP



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, LNCaP cells, A549 cells, NCI-H1299 cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66696-1-Ig targets ATP5O in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5O fusion protein Ag1458
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Calculated molecular weight 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC021233
Gene symbol ATP5O
Gene ID (NCBI) 539
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.

Protocols

Product Specific Protocols
WB protocol for ATP5O antibody 66696-1-IgDownload protocol
IHC protocol for ATP5O antibody 66696-1-IgDownload protocol
IF protocol for ATP5O antibody 66696-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols