ATP6V1B1 Polyclonal antibody
ATP6V1B1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14780-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human kidney tissue, human brain tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IF | See 1 publications below |
Product Information
14780-1-AP targets ATP6V1B1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ATP6V1B1 fusion protein Ag6332 |
Full Name | ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 |
Calculated molecular weight | 57 kDa |
Observed molecular weight | 56 kDa |
GenBank accession number | BC063411 |
Gene symbol | ATP6V1B1 |
Gene ID (NCBI) | 525 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for ATP6V1B1 antibody 14780-1-AP | Download protocol |
IHC protocol for ATP6V1B1 antibody 14780-1-AP | Download protocol |
IF protocol for ATP6V1B1 antibody 14780-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Mol Life Sci The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. | ||
Dis Model Mech Expression of N471D strumpellin leads to defects in the endolysosomal system. | ||
J Cell Physiol Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts. |