Tested Applications
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue, human brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14780-1-AP targets ATP6V1B1/2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, c.elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6332 Product name: Recombinant human ATP6V1B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-327 aa of BC063411 Sequence: MAMEIDSRPGGLPGSSCNLGAAREHMQAVTRNYITHPRVTYRTVCSVNGPLVVLDRVKFAQYAEIVHFTLPDGTQRSGQVLEVAGTKAIVQVFEGTSGIDARKTTCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPVVMAEDFLDINGQPINPHSRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKAVLDYHDDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLAT Predict reactive species |
| Full Name | ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC063411 |
| Gene Symbol | ATP6V1B1 |
| Gene ID (NCBI) | 525 |
| RRID | AB_2062378 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15313 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa. This antibody can cross-react with ATP6V1B2 due to the high homology between ATP6V1B1 and ATP6V1B2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP6V1B1/2 antibody 14780-1-AP | Download protocol |
| IHC protocol for ATP6V1B1/2 antibody 14780-1-AP | Download protocol |
| WB protocol for ATP6V1B1/2 antibody 14780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ATP6V1B1/2 Polyclonal antibody (Cat# 14780-1-AP) has 11 citations published in journals including J Am Soc Nephrol, Environ Int, Research, Cell Mol Life Sci, Mol Cell Biochem, J Biol Chem, J Cell Physiol, Dis Model Mech, Physiol Rep, and medRxiv. Research fields span renal intercalated cell differentiation and acid-base regulation, V-ATPase assembly, mitochondrial function and neurotoxicity, ovarian cancer progression, lysosomal lipid metabolism, endolysosomal defects, and urinary tract physiology.
| Species | Application | Title |
|---|---|---|
Research (Wash D C) Single-Cell RNA Sequencing Analysis Reveals the Role of Macrophage-Mediated CD44-AKT-CCL2 Pathways in Renal Tubule Injury during Calcium Oxalate Crystal Formation | ||
Environ Int Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury | ||
J Am Soc Nephrol Foxp1 Is Required for Renal Intercalated Cell Differentiation and Acid-Base Regulation | ||
J Am Soc Nephrol Hmx2 and Dmrt2 Coordinate the Differentiation of Intercalated Cell Subtypes in Kidney. | ||
Cell Mol Life Sci The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. | ||
Mol Cell Biochem ATP6V1B1 regulates ovarian cancer progression and cisplatin sensitivity through the mTOR/autophagy pathway
|
