ATP6V1B1 Polyclonal antibody

ATP6V1B1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14780-1-AP


ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3

Tested Applications

Positive WB detected inmouse kidney tissue, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14780-1-AP targets ATP6V1B1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1B1 fusion protein Ag6332
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC063411
Gene Symbol ATP6V1B1
Gene ID (NCBI) 525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of1 ATP6V1B1 is 57 kDa.


Product Specific Protocols
WB protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
IHC protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
IF protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Mol Life Sci

The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.

Authors - Enrico Castroflorio

Dis Model Mech

Expression of N471D strumpellin leads to defects in the endolysosomal system.

Authors - Lin Song

J Cell Physiol

Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts.

Authors - Milena Kluge

Environ Int

Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury

Authors - Xing Zhang

Mol Cell Biochem

ATP6V1B1 regulates ovarian cancer progression and cisplatin sensitivity through the mTOR/autophagy pathway

Authors - Shien Mo
  • KD Validated

Physiol Rep

Expression and localization of the mechanosensitive/osmosensitive ion channel TMEM63B in the mouse urinary tract

Authors - Marianela G Dalghi