ATP6V1B1 Polyclonal antibody

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  WB analysis using 14780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14780-1-AP (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14780-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14780-1-AP (ATP6V1B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14780-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14780-1-AP (ATP6V1B1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14780-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14780-1-AP (ATP6V1B1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 14780-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (14780-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ATP6V1B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14780-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, rat brain tissue, human brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

14780-1-AP targets ATP6V1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V1B1 fusion protein Ag6332
• Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC063411
• Gene Symbol: ATP6V1B1
• Gene ID (NCBI): 525
• RRID: AB_2062378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15313
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
mouse	IF	Dis Model Mech; Expression of N471D strumpellin leads to defects in the endolysosomal system.; Authors - Lin Song
human	WB	J Cell Physiol; Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts.; Authors - Milena Kluge
mouse	WB	Environ Int; Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury; Authors - Xing Zhang
human	WB,IHC	Mol Cell Biochem; ATP6V1B1 regulates ovarian cancer progression and cisplatin sensitivity through the mTOR/autophagy pathway; Authors - Shien Mo ; KD Validated
mouse	IF	Physiol Rep; Expression and localization of the mechanosensitive/osmosensitive ion channel TMEM63B in the mouse urinary tract; Authors - Marianela G Dalghi