ATP6V1B1 Polyclonal antibody

ATP6V1B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14780-1-AP

Synonyms

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1



Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, rat brain tissue, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14780-1-AP targets ATP6V1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1B1 fusion protein Ag6332
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC063411
Gene Symbol ATP6V1B1
Gene ID (NCBI) 525
RRIDAB_2062378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

Protocols

Product Specific Protocols
WB protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
IHC protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
IF protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Life Sci

The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.

Authors - Enrico Castroflorio
mouseIF

Dis Model Mech

Expression of N471D strumpellin leads to defects in the endolysosomal system.

Authors - Lin Song
humanWB

J Cell Physiol

Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts.

Authors - Milena Kluge
mouseWB

Environ Int

Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury

Authors - Xing Zhang
humanWB,IHC

Mol Cell Biochem

ATP6V1B1 regulates ovarian cancer progression and cisplatin sensitivity through the mTOR/autophagy pathway

Authors - Shien Mo
  • KD Validated
mouseIF

Physiol Rep

Expression and localization of the mechanosensitive/osmosensitive ion channel TMEM63B in the mouse urinary tract

Authors - Marianela G Dalghi