Mouse Afp Recombinant monoclonal antibody, PBS Only (Detector)

• 

  WB analysis of mouse placenta using 83367-2-RR (same clone as 83367-2-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 83367-2-RR (Afp antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83367-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00395-4

  Sandwich ELISA standard curve of MP00395-4, Mouse AFP Monoclonal Matched Antibody Pair - PBS only. 83367-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0670. 83367-2-PBS was HRP conjugated as the detection antibody. Range: 78.125-5000 pg/mL.

• 

  Sample test of MP00395-4

  Serum of sixteen individual healthy mice were measured. The mouse AFP concentration of detected samples was determined to be113.4 ng/mL with a range of 66.5-245.7 ng/mL.

• 

  Affinity and Kinetic Characterization of 83367-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83367-2-RR against Mouse Afp were performed. The affinity constant is 1.69 nM.

Afp Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83367-2-PBS

Clone No.240232C7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

240232C7, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein

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Product Information

83367-2-PBS targets Afp as part of a matched antibody pair:

MP00395-4: 83367-5-PBS capture and 83367-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0670

  Product name: Recombinant mouse Afp protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 19-605 aa of NM_007423.4

  Sequence: KALHENEFGIASTLDSSQCVTEKNVLSIATITFTQFVPEATEEEVNKMTSDVLAAMKKNSGDGCLESQLSVFLDEICHETELSNKYGLSGCCSQSGVERHQCLLARKKTAPASVPPFQFPEPAESCKAHEENRAVFMNRFIYEVSRRNPFMYAPAILSLAAQYDKVVLACCKADNKEECFQTKRASIAKELREGSMLNEHVCSVIRKFGSRNLQATTIIKLSQKLTEANFTEIQKLALDVAHIHEECCQGNSLECLQDGEKVMTYICSQQNILSSKIAECCKLPMIQLGFCIIHAENGVKPEGLSLNPSQFLGDRNFAQFSSEEKIMFMASFLHEYSRTHPNLPVSVILRIAKTYQEILEKCSQSGNLPGCQDNLEEELQKHIEESQALSKQSCALYQTLGDYKLQNLFLIGYTRKAPQLTSAELIDLTGKMVSIASTCCQLSEEKWSGCGEGMADIFIGHLCIRNEASPVNSGISHCCNSSYSNRRLCITSFLRDETYAPPPFSEDKFIFHKDLCQAQGKALQTMKQELLINLVKQKPELTEEQLAAVTADFSGLLEKCCKAQDQEVCFTEEGPKLISKTRDALGV

• Full Name: alpha fetoprotein
• Calculated Molecular Weight: 67KD
• Observed Molecular Weight: 67-72 kDa
• GenBank Accession Number: NM_007423.4
• Gene Symbol: Afp
• Gene ID (NCBI): 11576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02772
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.