Afp Recombinant monoclonal antibody

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  WB analysis of mouse placenta using 83367-2-RR

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 83367-2-RR (Afp antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83367-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83367-2-RR against Mouse Afp were performed. The affinity constant is 1.69 nM.

Afp Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83367-2-RR

Clone No.240232C7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, 240232C7

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Tested Applications

• Positive WB detected in: mouse placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83367-2-RR targets Afp in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: alpha fetoprotein
• Calculated Molecular Weight: 67KD
• Observed Molecular Weight: 67-72 kDa
• GenBank Accession Number: NM_007423.4
• Gene Symbol: Afp
• Gene ID (NCBI): 11576
• RRID: AB_3671021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02772
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.