Alpha Actinin Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 11313-2-AP (same clone as 11313-2-PBS)

  WB result of Alpha Actinin antibody (11313-2-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Actinin transfected HeLa cells. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  WB analysis using 11313-2-AP (same clone as 11313-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  WB analysis using 11313-2-AP (same clone as 11313-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 11313-2-AP (same clone as 11313-2-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 11313-2-AP (same clone as 11313-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11313-2-AP (Alpha Actinin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11313-2-AP (alpha Actinin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66219-1-Ig (N-cadherin) in red. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  IF Staining of HSkMC using 11313-2-AP (same clone as 11313-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 11313-2-AP (same clone as 11313-2-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (11313-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Alpha Actinin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Cat No. 11313-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

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Product Information

11313-2-PBS targets Alpha Actinin in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1859

  Product name: Recombinant human a-Actinin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 543-892 aa of BC015766

  Sequence: EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

• Full Name: actinin, alpha 1
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC015766
• Gene Symbol: Alpha Actinin
• Gene ID (NCBI): 87
• RRID: AB_2223815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12814
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.