Alpha Actinin Polyclonal antibody

Alpha Actinin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11313-2-AP

Synonyms

actinin, alpha 1, ACTN1, alpha Actinin, Alpha actinin 1, F actin cross linking protein, Non muscle alpha actinin 1



Tested Applications

Positive WB detected inNIH/3T3 cells, mouse spleen tissue, rat heart tissue, C6 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, NIH/3T3 cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11313-2-AP targets Alpha Actinin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag1859
Full Name actinin, alpha 1
Calculated molecular weight 103 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC015766
Gene symbol ACTN1
Gene ID (NCBI) 87
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
WB protocol for Alpha Actinin antibody 11313-2-APDownload protocol
IHC protocol for Alpha Actinin antibody 11313-2-APDownload protocol
IF protocol for Alpha Actinin antibody 11313-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Transl Res

Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.

Authors - Pengyu Jia
mouseWB

Aging (Albany NY)

Aging-dependent loss of GAP junction proteins Cx46 and Cx50 in the fiber cells of human and mouse lenses accounts for the diminished coupling conductance.

Authors - Xiao-Dong Gong
ratWB

Biomed Pharmacother

Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats.

Authors - Yao Wenjuan W
mouseWB

Biochem Biophys Res Commun

Sirtuin 6 ameliorates alcohol-induced liver injury by reducing endoplasmic reticulum stress in mice.

Authors - Yue Xin
mouseWB

Biochem Biophys Res Commun

Adipocyte-specific deletion of Depdc5 exacerbates insulin resistance and adipose tissue inflammation in mice.

Authors - Jinghong Zhang
mouseIHC

Exp Eye Res

Dynamic spatiotemporal expression pattern of limbal stem cell putative biomarkers during mouse development.

Authors - Zhi Hou Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jason (Verified Customer) (10-28-2019)

Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Mouse myocardium
Alpha Actinin Antibody Immunohistochemistry, validation (1/100 dilution) in Mouse myocardium (Cat no:11313-2-AP)
FH

Steeve (Verified Customer) (01-08-2018)

Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1'1000
  • Cell Tissue Type: human umbilical veins