|Positive WB detected in||NIH/3T3 cells, mouse spleen tissue, rat heart tissue, C6 cells|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue, NIH/3T3 cells, C2C12 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11313-2-AP targets Alpha Actinin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alpha Actinin fusion protein Ag1859|
|Full Name||actinin, alpha 1|
|Calculated molecular weight||103 kDa|
|Observed molecular weight||100-105 kDa|
|GenBank accession number||BC015766|
|Gene ID (NCBI)||87|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jason (Verified Customer) (10-28-2019)
Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.
Steeve (Verified Customer) (01-08-2018)
Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin