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Alpha Actinin Polyclonal antibody

Alpha Actinin Polyclonal Antibody for WB, IF, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 11313-2-AP

Synonyms

actinin, alpha 1, ACTN1, alpha Actinin, Alpha actinin 1, F actin cross linking protein, Non muscle alpha actinin 1



Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, mouse spleen tissue, rat heart tissue, C6 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat heart tissue, mouse heart tissue
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11313-2-AP targets Alpha Actinin in WB, IF, IHC, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag1859
Full Name actinin, alpha 1
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC015766
Gene Symbol Alpha Actinin
Gene ID (NCBI) 87
RRIDAB_2223815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
WB protocol for Alpha Actinin antibody 11313-2-APDownload protocol
IHC protocol for Alpha Actinin antibody 11313-2-APDownload protocol
IF protocol for Alpha Actinin antibody 11313-2-APDownload protocol
FC protocol for Alpha Actinin antibody 11313-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Redox Biol

MK-886 protects against cardiac ischaemia/reperfusion injury by activating proteasome-Keap1-NRF2 signalling

Authors - Kai-Na Shi
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
humanIF

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
mouseWB

Cell Death Differ

Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.

Authors - Ming Zou
mouseIF

Transl Res

Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.

Authors - Pengyu Jia
mouseWB

Theranostics

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Authors - Jing Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jason (Verified Customer) (10-28-2019)

Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Mouse myocardium
Alpha Actinin Antibody Immunohistochemistry, validation (1/100 dilution) in Mouse myocardium (Cat no:11313-2-AP)
FH

Steeve (Verified Customer) (01-08-2018)

Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1'1000
  • Cell Tissue Type: human umbilical veins