AuthentiKine™ Human Growth Hormone ELISA Kit
Cat no : KE00221
Synonyms
GH,GH1,GH 1,GH-N,Growth hormone
Validation Data Gallery
Product Information
KE00221 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ELISA kit is to be used to detect and quantify protein levels of endogenous HGH. The assay recognizes human HGH. An antibody specific for HGH has been pre-coated onto the microwells. The HGH protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HGH is added to detect the captured HGH protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human Growth Hormone ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2688 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 82% | 73%-91% |
| Cell culture supernatants | 80% | 73%-91% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 109.2 | 2.1 | 1.9 |
| 2 | 20 | 24.2 | 0.7 | 2.7 |
| 3 | 20 | 5.3 | 0.3 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 103.4 | 7.6 | 7.3 |
| 2 | 24 | 23.2 | 1.8 | 7.9 |
| 3 | 24 | 13.5 | 0.6 | 4.8 |
Background Information
Growth hormone (GH) is a heterogeneous polypeptide consisting of a number of different isoforms and variants. Its secretion from pituitary somatotrophs is pulatile, with low or undetectable basal levels between peaks. GH secretion has a characteristic diurnal pattern with maximal levels recorded during sleep. Growth hormone is a powerful anabolic hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. The actions of GH are mediated by GH receptors (GHR), which are widely distributed among tissues. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GH,GH1,GH 1,GH-N,Growth hormone |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Commun Splicing diversity enhances the molecular classification of pituitary neuroendocrine tumors | ||
Sci Transl Med Amino acid supplementation enhances in vivo efficacy of lipid nanoparticle-mediated mRNA delivery in preclinical models. | ||
Dis Markers Effect of Vitamin D Combined with Recombinant Human Growth Hormone in Children with Growth Hormone Deficiency. |