BCL6 Recombinant antibody
BCL6 Recombinant Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3K6
Cat no : 81754-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Ramos cells, Daudi cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | Ramos cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Product Information
81754-1-RR targets BCL6 in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | BCL6 fusion protein Ag15519 |
Full Name | B-cell CLL/lymphoma 6 |
Calculated molecular weight | 706 aa, 79 kDa |
Observed molecular weight | 79-95 kDa |
GenBank accession number | BC150184 |
Gene symbol | BCL6 |
Gene ID (NCBI) | 604 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.
Protocols
Product Specific Protocols | |
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WB protocol for BCL6 antibody 81754-1-RR | Download protocol |
IHC protocol for BCL6 antibody 81754-1-RR | Download protocol |
FC protocol for BCL6 antibody 81754-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |