|Positive WB detected in||HeLa cells, human brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10476-1-AP targets BRD8 in WB, IP, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||BRD8 fusion protein Ag0790|
|Full Name||bromodomain containing 8|
|Calculated molecular weight||120 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC008039|
|Gene ID (NCBI)||10902|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].
Arch Biochem Biophys
BRD8, which is negatively regulated by miR-876-3p, promotes the proliferation and apoptosis resistance of hepatocellular carcinoma cells via KAT5.
ANP32E is a histone chaperone that removes H2A.Z from chromatin.