Beta-2-Microglobulin Monoclonal antibody

Beta-2-Microglobulin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human and More (1)







Cat no : 66207-1-Ig


B2M, Beta-2-microglobulin, HDCMA22P

Tested Applications

Positive WB detected inA431 cells, Raji cells, LNCaP cells
Positive IHC detected inhuman prostate cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman prostate cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66207-1-Ig targets Beta-2-Microglobulin in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name beta-2-microglobulin
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.


Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
IHC protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
IF protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Immunother Cancer

Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinoma

Authors - Jihua Tian


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Santhosh (Verified Customer) (04-15-2019)

Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
Beta-2-Microglobulin Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66207-1-Ig)