Tested Applications
| Positive WB detected in | A431 cells, Raji cells, LNCaP cells |
| Positive IHC detected in | human prostate cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human prostate cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66207-1-Ig targets Beta-2-Microglobulin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | beta-2-microglobulin |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC032589 |
| Gene Symbol | B2M |
| Gene ID (NCBI) | 567 |
| ENSEMBL Gene ID | ENSG00000166710 |
| RRID | AB_2881598 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61769 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Beta-2-Microglobulin antibody 66207-1-Ig | Download protocol |
| IHC protocol for Beta-2-Microglobulin antibody 66207-1-Ig | Download protocol |
| WB protocol for Beta-2-Microglobulin antibody 66207-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Beta-2-Microglobulin Monoclonal antibody (Cat# 66207-1-Ig) has 5 total citations. These appear in J Immunother Cancer, J Agric Food Chem, Int Immunopharmacol (×2), and J Virol. The associated research fields include immune-combination therapy in renal cell carcinoma, antigen processing and presentation via ERAP1 inhibition, neutrophil extracellular traps in kidney transplant rejection, and FcRn-mediated viral uncoating. All citations used the antibody for Western blot applications across mouse, human, rat, and monkey species.
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinoma | ||
J Agric Food Chem Carnosic Acid: A Novel Selective Inhibitor of ERAP1 by Direct Binding and Its Modulation of Antigen Processing and Presentation | ||
Int Immunopharmacol Corilagin regulates antigen processing and presentation by directly binding to inhibit ERAP1. | ||
Int Immunopharmacol Identification of potential targets regulating neutrophil extracellular traps in acute rejection of kidney transplantation based on transcriptomics and animal experiments | ||
J Virol The neonatal Fc receptor (FcRn) is required for porcine reproductive and respiratory syndrome virus uncoating |
Reviews
What customers say
The single review for this product is a 5-star rating from an immunohistochemistry application. The reviewer used a 1:200 dilution on human tonsil FFPE tissue, with rehydration and antigen retrieval performed using citrate buffer. The result was positive, as indicated by the accompanying image. Overall, the feedback is favorable, confirming the antibody's performance in IHC at the stated dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Santhosh (Verified Customer) (04-15-2019) | Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.
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