Beta-2-Microglobulin Monoclonal antibody

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  WB analysis of Raji using 66207-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig (B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66207-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 66207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 66207-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human prostate cancer using 66207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Beta-2-Microglobulin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66207-1-Ig

Clone No.1C3B7

Publications(3)

Reviews (1)

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Synonyms

B2M, 1C3B7, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

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Tested Applications

• Positive WB detected in: A431 cells, Raji cells, LNCaP cells
• Positive IHC detected in: human prostate cancer tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human prostate cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

66207-1-Ig targets Beta-2-Microglobulin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• ENSEMBL Gene ID: ENSG00000166710
• RRID: AB_2881598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61769
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Publications

Species	Application	Title
mouse	WB	J Immunother Cancer; Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinoma; Authors - Jihua Tian
human	WB	J Agric Food Chem; Carnosic Acid: A Novel Selective Inhibitor of ERAP1 by Direct Binding and Its Modulation of Antigen Processing and Presentation; Authors - Jiaqi Wu
monkey	WB	J Virol; The neonatal Fc receptor (FcRn) is required for porcine reproductive and respiratory syndrome virus uncoating; Authors - Kang Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Santhosh (Verified Customer) (04-15-2019)

Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

Beta-2-Microglobulin Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66207-1-Ig)