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Beta-2-Microglobulin Monoclonal antibody

Beta-2-Microglobulin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C3B7

Cat no : 66207-1-Ig

Synonyms

B2M, Beta-2-microglobulin, HDCMA22P

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    WB analysis of Raji using 66207-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig (B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human prostate cancer using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human prostate cancer using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human renal cell carcinoma using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human renal cell carcinoma using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IF Staining of human prostate cancer using 66207-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman prostate cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman prostate cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66207-1-Ig targets Beta-2-Microglobulin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name beta-2-microglobulin
Calculated molecular weight 14 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protocols

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
IHC protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
IF protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Santhosh (Verified Customer) (04-15-2019)

Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
Beta-2-Microglobulin Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66207-1-Ig)