Beta-2-Microglobulin Monoclonal antibody
Beta-2-Microglobulin Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
WB, IHC, IF, ELISA
Cat no : 66207-1-Ig
Validation Data Gallery
|Positive WB detected in||Raji cells|
|Positive IHC detected in||human prostate cancer tissue, human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human prostate cancer tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66207-1-Ig targets Beta-2-Microglobulin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2b|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||13 kDa|
|GenBank accession number||BC032589|
|Gene ID (NCBI)||567|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
|Product Specific Protocols|
|WB protocol for Beta-2-Microglobulin antibody 66207-1-Ig||Download protocol|
|IHC protocol for Beta-2-Microglobulin antibody 66207-1-Ig||Download protocol|
|IF protocol for Beta-2-Microglobulin antibody 66207-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Santhosh (Verified Customer) (04-15-2019)
Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.