CD38 Polyclonal antibody

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  WB analysis using 25284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 25284-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 25284-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD38 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25284-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Tested Applications

• Positive WB detected in: Raji cells, HL-60 cells, Daudi cells, Ramos cells
• Positive IHC detected in: human tonsillitis tissue, human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

25284-1-AP targets CD38 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD38 fusion protein Ag17868
• Full Name: CD38 molecule
• Calculated Molecular Weight: 300 aa, 34 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC007964
• Gene Symbol: CD38
• Gene ID (NCBI): 952
• ENSEMBL Gene ID: ENSG00000004468
• RRID: AB_2880007
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28907
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Oncol; CD38 Multi-Functionality in Oral Squamous Cell Carcinoma: Prognostic Implications, Immune Balance, and Immune Checkpoint.; Authors - Zhuang Ding
human	IF	Cell Commun Signal; TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma.; Authors - Hua Bai
human	WB,IHC	Oncol Rep; CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer.; Authors - Shan Liao
human	IHC	Heliyon; Diagnostic, prognostic, and immunological roles of CDSN in ovarian cancer; Authors - Huan Zeng
mouse	WB,IF	Cell Rep; Tak1 licenses mitochondrial transfer from astrocytes to POMC neurons to maintain glucose and cholesterol homeostasis; Authors - Kaili Yin
		J Cancer Res Clin Oncol; KIF22 promotes multiple myeloma progression by regulating the CDC25C/CDK1/cyclinB1 pathway; Authors - Meng Zhai