CD38 Polyclonal antibody

CD38 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25284-1-AP

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Validation Data Gallery

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  WB analysis using 25284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HL-60 using 25284-1-AP

  1X10^6 HL-60 cells were stained with 0.2ug CD38 antibody (25284-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: Raji cells, HL-60 cells, Daudi cells, Ramos cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25284-1-AP is CD38 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD38 fusion protein Ag17868
• Full Name: CD38 molecule
• Calculated molecular weight: 300 aa, 34 kDa
• Observed molecular weight: 40-45 kDa
• GenBank accession number: BC007964
• Gene symbol: CD38
• Gene ID (NCBI): 952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Publications

Species	Application	Title
human	WB,IHC	Oncol Rep; CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer.; Authors - Shan Liao
human	IF	Cell Commun Signal; TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma.; Authors - Hua Bai