PE Anti-Human CD45RA (HI100)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

HI100

Cat no : PE-65108

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Tested Applications

Positive FC detected inHuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl(0.06µg) per 10^6 cells in a 100 µl suspension or 5 µl(0.06µg) per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

PE-65108 targets CD45RA in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496, 565 nm/578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
FC protocol for PE CD45RA antibody PE-65108Download protocol
Standard Protocols
Click here to view our Standard Protocols