APC Anti-Human CD45RA (HI100)

CD45RA Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC

Conjugate

APC Fluorescent Dye

CloneNo.

HI100

Cat no : APC-65108

Synonyms

B220, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Tested Applications

Positive IF detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:150-1:600
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

APC-65108 targets CD45RA in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
IF protocol for APC CD45RA antibody APC-65108Download protocol
FC protocol for APC CD45RA antibody APC-65108Download protocol
Standard Protocols
Click here to view our Standard Protocols