APC Anti-Human CD45RA (HI100)

CD45RA Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

IF/ICC, FC

Conjugate

APC Fluorescent Dye

CloneNo.

HI100

Cat no : APC-65108

Synonyms

CD45, B220, L CA, HI100, GP180

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Validation Data Gallery

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  IF Staining of human peripheral blood mononuclear cells using APC-65108

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using APC Anti-Human CD45RA (APC-65108, Clone: HI100) and CoraLite®488-conjugated Anti-Human CD45RO (CL488-65150, Clone: UCHL1) at dilution of 1:300.

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  FC experiment of human PBMCs using APC-65108

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45RO and 5 ul APC Anti-Human CD45RA (HI100) (APC-65108, Clone: HI100) or APC Mouse IgG2b Isotype Control (MPC-11) (APC-65128, Clone: MPC-11). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using APC-65108

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45RA (HI100) (APC-65108, Clone: HI100) (red) or APC Mouse IgG2b Isotype Control (MPC-11) (APC-65128, Clone: MPC-11) (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive IF/ICC detected in: human peripheral blood mononuclear cells
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-65108 targets CD45RA in IF/ICC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• RRID: AB_2882997
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).