CoraLite® Plus 488 Anti-Human CD45RA (HI100)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HI100

Cat no : CL488-65108

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Validation Data Gallery

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  FC experiment of human peripheral blood lymphocytes using CL488-65108

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD45RO (APC-65150, Clone: UCHL1) and 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD45RA (CL488-65108, Clone: HI100). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL488-65108

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD45RO (APC-65150, Clone: UCHL1) and CoraLite® Plus 488-conjugated mouse IgG2b isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65108 targets CD45RA in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: PTPRC
• Gene ID (NCBI): 5788
• RRID: AB_2883182
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).