Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody

• 

  FC experiment of human PBMCs using 65593-1-MR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone: KP1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

• 

  FC experiment of human PBMCs using 65593-1-MR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

• 

  FC experiment of human PBMCs using 65593-1-MR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (left) or 0.25 ug Anti-Human CD68 (65202-1-Ig, Clone: KP1) (right) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

CD68 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 65593-1-MR

Clone No.KP1

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Synonyms

CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1

---

Tested Applications

• Positive FC (Intra) detected in: human PBMCs
• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65593-1-MR targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• RRID: AB_3670342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.