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Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody (65593-1-MR)

Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody

CD68 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 65593-1-MR

Clone No. KP1

Publications (1)

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Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Synonyms

CD68 molecule, GP110, KP1, Macrosialin, SCARD1

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Biotin CoraLite® Plus 405 CoraLite® Plus 488 CoraLite® Plus 647 CoraLite® Plus 750 CoraLite®594 CoraLite®700 CoraLux™ Violet 510 FITC Plus

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Filter: AllFC (Intra)

FC experiment of human PBMCs using 65593-1-MR
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone: KP1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Monocytes were gated.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone: KP1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Monocytes were gated.
FC experiment of human PBMCs using 65593-1-MR
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Monocytes were gated.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Monocytes were gated.
FC experiment of human PBMCs using 65593-1-MR
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (left) or 0.25 ug Anti-Human CD68 (65202-1-Ig, Clone: KP1) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Monocytes were gated.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (left) or 0.25 ug Anti-Human CD68 (65202-1-Ig, Clone: KP1) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Monocytes were gated.

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What's the best dilution for CD68 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive FC (Intra) detected in

human PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65593-1-MR targets CD68 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Recombinant
Type	Antibody
Immunogen	N/A Predict reactive species
Full Name	CD68 molecule
Calculated Molecular Weight	37 kDa
GenBank Accession Number	BC015557
Gene Symbol	CD68
Gene ID (NCBI)	968
RRID	AB_3670342
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P34810
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2-8°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
FC protocol for CD68 antibody 65593-1-MR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody (Cat# 65593-1-MR) has 1 citation. It appears in Oncogenesis (IF: 6), a 2026 publication studying how lung adenocarcinoma cell-derived lactate activates cancer-associated fibroblasts to promote macrophage M2 polarization via exosomal miR-214-5p. The research field is oncology, specifically tumor microenvironment and immune cell crosstalk in lung adenocarcinoma.

All (1)

Species	Application	Title
		Oncogenesis Lung adenocarcinoma cell-derived lactate activates CAFs to promote macrophage M2 polarization via exosomal miR-214-5p. Authors - Leilei Tao View Article