Tested Applications
| Positive FC (Intra) detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL405-65593 targets CD68 in FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | CD68 molecule |
| Calculated Molecular Weight | 37 kDa |
| GenBank Accession Number | BC015557 |
| Gene Symbol | CD68 |
| Gene ID (NCBI) | 968 |
| RRID | AB_3747101 |
| Conjugate | CoraLite® Plus 405 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
| Excitation Laser | Violet Laser (405 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P34810 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD68 antibody CL405-65593 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL405-65593 is an Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody. It has a total of 7 citations associated with the parent product. One verified citation appears in Phytotherapy Research (Impact Factor 8.1), published in 2026, studying resveratrol's role in ameliorating hypertrophic scar formation via ASIC3-mediated fibroblast-macrophage crosstalk. The research field encompasses wound healing, fibrosis, and macrophage immunology, with flow cytometry used as the application.
| Species | Application | Title |
|---|---|---|
Phytother Res Resveratrol Ameliorates Hypertrophic Scar Formation by Regulating ASIC3-Mediated Fibroblast-Macrophage Crosstalk: A Mechanistic Study. |
