CD96 Recombinant monoclonal antibody

• 

  WB analysis using 85439-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85439-7-RR (CD96 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CD96 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85439-7-RR

Clone No.242964E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD96 molecule, Cell surface antigen CD96, T cell surface protein tactile, T cell-activated increased late expression protein, TACTILE

---

Tested Applications

• Positive WB detected in: A375 cells, MOLT-4 cells, HuT 78 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85439-7-RR targets CD96 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3520

  Product name: Recombinant Human CD96 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-503 aa of NM_198196

  Sequence: VWEKTVNTEENVYATLGSDVNLTCQTQTVGFFVQMQWSKVTNKIDLIAVYHPQYGFYCAYGRPCESLVTFTETPENGSKWTLHLRNMSCSVSGRYECMLVLYPEGIQTKIYNLLIQTHVTADEWNSNHTIEIEINQTLEIPCFQNSSSKISSEFTYAWSVEDNGTQETLISQNHLISNSTLLKDRVKLGTDYRLHLSPVQIFDDGRKFSCHIRVGPNKILRSSTTVKVFAKPEIPVIVENNSTDVLVERRFTCLLKNVFPKANITWFIDGSFLHDEKEGIYITNEERKGKDGFLELKSVLTRVHSNKPAQSDNLTIWCMALSPVPGNKVWNISSEKITFLLGSEISSTDPPLSVTESTLDTQPSPASSVSPARYPATSSVTLVDVSALRPNTTPQPSNSSMTTRGFNYPWTSSGTDTKKSVSRIPSETYSSSPSGAGSTLHDNVFTSTARAFSEVPTTANGSTKTNHVHITGIVVNKPKDGM

• Full Name: CD96 molecule
• Calculated Molecular Weight: 402 aa, 46 kDa
• Observed Molecular Weight: 160
• GenBank Accession Number: NM_198196
• Gene Symbol: CD96
• Gene ID (NCBI): 10225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P40200
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD96 is a member of the poliovirus receptor (PVR, CD155)-nectin family that includes T cell Ig and ITIM domain (TIGIT) and CD226.. CD96 expression is broadly similar between mice and humans, and is present on a proportion of hematopoietic stem cells, αβ and γδ T-cells, NK cells and a sub-population of B-cells in humans and present on αβ and γδ T-cells, NK cells and NKT cells in mice (PMID: 32043568, 29531070)